Scott Morrison said he "feared for the worst" after the car crash involving members of his security team in Tasmania on Thursday.
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Mr Morrison has spoken to the two federal police officers involved in the crash which temporarily halted his federal election campaign.
One officer sustained serious injuries and remains in hospital, while another has been discharged.
The four police officers were rushed to Launceston General Hospital after their car collided with another vehicle outside Elizabeth Town, roughly halfway between Devonport and Launceston, on Thursday afternoon.
Mr Morrison was not involved in the accident, which occurred behind his vehicle. Campaign events scheduled for the remainder of Thursday were cancelled following the crash.
The Prime Minister provided the update after attending a Good Friday service in the Melbourne electorate of Chisholm, which is held by Liberal Gladys Liu on a margin of just 0.5 per cent.
"It was a terrible accident," Mr Morrison told reporters at Syndal Baptist Church.
'It's a reminder that, you know, we've got to be safe on our roads as we're moving around.
"I want to thank everybody down there at the Launceston General Hospital.
"I wanted to thank all the first responders too. The emergency services responded incredibly quickly yesterday, getting there and I want to thank those who are at the scene as well, who helped our officers and who went to support as well at that time.
"Thankfully everyone is doing a lot better, but it was obviously a very concerning moment."
Labor leader Anthony Albanese
Good Friday marks a pause in the federal election campaign, with the two leaders putting politics aside to observe the Christian holiday.
Mr Morrison, who is a Pentecostal Christian, said of his faith: "It informed me, it encouraged me, guided me over my entire life".
Labor leader Anthony Albanese this morning attended a service at St Charbel's Maronite church in the Western Sydney suburb of Punchbowl.
The Labor leader met with Father Superior Charbel Abboud and laid flowers on a coffin, which represents Jesus' resting place.
Flanked by Labor figures Tony Burke and Jason Clare, Mr Albanese listened to hymns and passages in both Arabic and English.
Across the aisle, also in the front row, was the Prime Minister's wife, Jenny Morrison, and his two children.
Former Prime Minister Tony Abbott, who is a regular attendee of the Good Friday service at St Charbel's Monastery, was seated a few seats across from the Morrison.
The Good Friday pause has come at the end of a rocky start to the federal election campaign for the two leaders
Mr Albanese has been trying to recover from an embarrassing opening day gaffe where he failed to name the unemployment rate or cash rate.
Mr Morrison faced his toughest day on Thursday after walking back the Coalition's commitment to establish a federal anti-corruption watchdog.